| Son of Salt (SOS) Pond novel isolate, Peribacillus frigotolerans, from a hypersaline pond located in the McMurdo Ice Shelf in Antarctica has shown to produce proteases and βgalactosidases. We have identified three proteases (serine protease 1, protease 5, and zinc protease 2) and designed PCR primers to amplify the genes. Utilizing the aLICator cloning system, we were able to clone the three gene targets. Additionally, we have transformed recombinant plasmid DNA into an expression host and preliminary expression experiments have been conducted. |